| Time | Topic : Details |
|---|---|
| 9:00 | Welcome / Housekeeping (Chris & Marci) |
| 9:30 | Orienting on scRNA-Seq (Chris) |
| Getting started with Seurat (Raymond) | |
| 11:00 | Break |
| 11:15 | Cell Ranger in Action (Chris) |
| Secondary QC & Filtering (Raymond) | |
| Normalization | |
| 1:00 | Day 1 concludes |
| 9:00 | Housekeeping, Review, and Questions (Chris) |
| 9:20 | Principal Component Analysis & Integration (Dana) |
| Clustering & Projection | |
| 11:00 | Break |
| 11:15 | An overview of different sequencing approaches (Tricia) |
| Clustering & Projection (continued) | |
| 1:00 | Day 2 concludes |
| 9:00 | Housekeeping, Review and Questions (Chris) |
| 9:20 | Marker identification & Visualization |
| Cell type annotation | |
| 11:00 | Break |
| Cell type annotation (continued) | |
| 11:15 | Differential expression analysis |
| 12:45 | Wrap-up (Chris) |
| 1:00 | Workshop concludes |