RNA-seq Demystified

Schedule

Day 1 (Chris & Travis)

Time Topic : Details
9:00 Introductions and Startup
9:30 Sequence QC:
    Warming Up
    Sequence QC
    Trimming
11:00 Break
11:15 Alignment:
    Reference Genomes
    Alignment
Quantification:
    Alignment QC and Quantification
    Additional Details
1:00 Day 1 concludes

Day 2 (Chris & Raymond)

9:00 Review and questions
9:20 Quantification (continued)
Part 2
Groups and Modeling:
    DE Introduction
    DE Initialization
    DE Normalization and Modeling
11:00 Break
11:15 Expression plots:
    Sample QC
1:00 Day 2 concludes

Day 3 (Raymond)

9:00 Review
9:10 Differential Expression:
    DE Testing
    DE Visualization and Annotation
11:00 Break
11:15     DE Visualization and Annotation (continued)
    Bonus Content
12:45 Wrap-up
1:00 Day 3 concludes